ISOLASI DAN KARAKTERISASI BACILLUS LIPOLITIK DARI TANAH KEBUN RAYA LIWA
Article Sidebar
Liwa Botanical Gardens,
lipolitics,
soil
Main Article Content
Abstract
Bacillus is a bacterium that has the ability to survive in unfavorable environmental conditions, one of which is in nutrient-poor soils such as the Liwa Botanical Gardens. Bacillus isolates have been obtained from the soil of the Liwa Botanical Gardens which have lipolytic abilities. This study aimed to determine the activity of lipolytic bacteria isolates from Bacillus from Liwa Botanical Gardens. In this study, Bacillus was isolated from the soil of the Liwa Botanical Gardens using a graded dilution method, using Nutrient Agar media. The Bacillus isolates obtained were then purified in Nutrient agar media. Bacillus isolates were inoculated in lipase test media with a composition of 0.01% methyl red, 10 g peptone, and Nutrient Agar, 5% sterile olive oil, 2.5% Tween-80, 5 g NaCl, 0.1 g CaCl2.2H2O. The results of this study obtained 5 isolates of Bacillus which had fairly good growth, namely isolates BP 13, BP 14, BP16, TMA 13, TSR 5. With the results obtained the largest lipolytic activity was isolate BP 16 with an average index value of 6.34 cm while the lowest lipolytic activity was TMA13 isolate with an average index value of 2,345 cm.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Bestari, C.D. (2015). Uji Kualitatif dan Kuantitatif Isolat Bakteri Lipolitik dari Limbah Cair Pabrik Pengolahan Ikan Kecamatan Muncar Banyuwangi. Biotropika jurnal of Tropical Biology, 3(3). 151-155.
Chairunnisa. (2019). Isolasi dan Uji Lipolitik dalam Mendegradasi Minyak pada Limbah Cair Kelapa Sawit di Kebun Merihat, Pematang Siantar. Medan: Skripsi. Program Studi Biologi. Universitas Medan Area.
Ervina, E., Sumardi, Ekowati, C. & Rosa, E. (2020). Lipolytic Screening of Bacillus Genera as Biocontrol Candidate in Coffee Plantation. Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati, 7(1). 31-34.
Fardiaz. (1992). Mikrobiologi Pangan I. Jakarta: Gramedia.
Gupta, R., & Rathi, P. (2003). Lipase Assay for Coventional and Molecular Screening an Overview. Biotechnol Appl Biochem, 37. 63-67.
Holt, G., Krieg, R., Sneath, P., Staley, J., & Williams, S. (1994). Bergey's Manual og Determinative. Philadelphia: A Worters Kluwer Company.
Melliawati, R. (2018). Penapisan Isolat Kapang Endofit Lipolitik untuk Produksi Lipase pada Ampas Kelapa .Jjurnal Biopral Industri, 2. 95-105.
Muharni, H. & Anggraini, M. (2015). Isolasi dan Indentifikasi Bakteri Termo-lipolitik dengan Pendekatan Biologi Molekuler berbasis Gen 16S rRNA. SEMIRATA, 4(1).
Nurdin, G., Mubarik, N. & Sudirman, L. (2015). Selection of Chitinolyytic Bacteria as Biocontrol of Colletotrichum capsici. Malaya Journal Microbiol, 12(1). 35-42.
Simamora, C. & Sukmawati, S. (2020). Identifikasi dan Karakterisasi Aktivitas Ekstrak Kasar Enzim Lipase Isolat Bakteri Lipolitik lptk 19 Asal Tempe biji Karet. Median Jurnal Ilmu Eksakta , 12(1). 28-37.
Sirisha, E., Rajasekar, N. & Narasu, M. (2010). Isolation and Optimization of Lipase Producing Bakteria from oil Contaminated Soils. Advances in Biological Research, 4(5). 249-252