EFEK UKURAN, WAKTU PEMANASAN DALAM PERTUMBUHAN DAN PERKEMBANGAN BAWANG PUTIH (Allium sativum L) SECARA INKONVENSIONAL
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garlic,
heat treatment,
meristem,
shoot tip
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Abstract
Garlic (Allium sativum L.) is included in the genus Allium sp., propagated vegetatively through clove. In plants that are propagated vegetative, viral systemic diseases will be carried over to the next generation. In conventional methods/ tissue culture techniques combined with size and heat treatment the explant material can used as virus elimination. The activity research conducted at the Balitsa tissue culture laboratory from April to July 2018. The purpose of the study was to see the effect of size, heat treatment of explant material on the growth and development of garlic variety Tawang Mangu. The treatments were (1) explant size E1 = 1/3 clove, E2 = shoot tip, E3 = meristem tissue, (2) heat treatment 37oC, T1 = without treatment, T2 = heat treatment for 2 weeks, T3 = heat treatment for 4 weeks, T4 = heat treatment 6 weeks. The composition of the growing media: MS + MS vits + sucrose 30 g/l + IAA 2 mg/l +Kinetin 2 mg/l + GA3 0.01 mg/l + Gel gro 2 g/l, pH 5.7. The results of the study (1) were visually observed that the smaller the size of the explants and the length of heat treatment, the lower the percentage of explants that grew normally. (2) Percentage of contamination 33.33 – 60% caused by bacteria or fungi. (3) The percentage of infected plantlet with virus with DAS ELISA technique were 40 – 66.67%.
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References
Abo El Nil, MM 1977. Organogenesis and embryo genesis in callus culture of garlic (Allium sativum L). Plant Sci. letter. 9; 259 - 264.
Armini, NM; GA Wattimena; L. Winata. 1992. Perbanyakan tanaman dalam bioteknologi tanaman I. Dalam Wattimena et al. (eds) PAU Bioteknologi IPB. Dirjen Dikti Depnt P&K, pp 12 – 18.
Awan A.R; Mughal S.M; Iftiehar Y and Khan H.Z. 2007. In vitro elimination potato leaf roll virus from potato varieties, Euro. J. Sci. Res. 18; 155 -164.
Badoni, A, and Chamka, J.S. 2010. In vitro sterilization protocol for micro propagation of Solanum tuberosum cv. Kufri Himalini. Academia Arena. 2 (4); pp 24-27.
Barandiara, X; Martin N; Rodriguez Conde, M.F; Pietro Adi M.J and Pietro, A 1999. An efficient method for callus culture and shoot regeneration of garlic (Allium sativum L). Hort. Sci. 34; 348 – 349.
Bertaccini A, Botti S, tabanelli D, Dradi G, Fogher C, Previati A, Re F, 2004 Micropropagation and establishment of mite-borner virus free garlic (Allim sativum L). Acta Hort. 631; 201-206
Conci.V.C.Canavelli A. Lunello P. Rienzo J.D. Nome S.F. Zumelzu G and Italia R. 2003. Yield Losses associated with virus – infected garlic plants during five successive years. Plant Dis. 87; 1411-1415.
Dixit, V and Chaudhary B.R.2013. Allium sativum: for step approach to efficient micropropagation. Int j of innovative Biological Research: 2; 6-14
Eady C; RC Bulter and Y Suo. 1998. Somatic embryogenesis and plant regeneration from immature embryo culture of onion (Allium cepa L). Plant Cell. Report. 18; 111- 116.
Geier T. 1990. Anthurium. In Ammirato, PV; DA Evans; WR Sharp and YPS Bajaj (eds). Handbook of plant cell culture orname ntal species. Mac Grow Hill. N. Y. vol 5; p 228 - 252.
George EF; Hall MA and de Klerk, GJ 2008. The component of plant tissue culture medium I, macro and micronutrients in (eds) George EF; Hall MA and de Klerk, GJ Plant propagation by tissue culture. The background vol I. 3 rd (eds). Springer Netherlands.p 274 - 338.
Gopal J. and Garg I.D. 2011. An efficient protocol of chemo theraphy for elimination of potato (Solanum tuberosum L) virus by meristem tip culture. Ind. J. Ag. Sci. 81; 544 – 549.
Gunaeni. N; Wulandari AW and Muharam A. 2011. Insiden penyakit tular umbi pada tiga belas varietas bawang merah asal Jabar dan Jateng. J. Hort. 21 (2); 164 - 172.
Haider S, Hossain M, Rahmans, Sultana S, Quddus T, Chakraborti, M Hoque, Shahriar M and Haque M. 2015. In vitro plantlet regeneration of four local garlic (Allium sativum L) accession of Bangladesh. British Biotech J. 8: 1- 12.
Hamidah M; A.G. Karim and P.C. Debergh. 1997. Somatic embryogenesis and plant regeneration Anthurium scherzerianum. Plant cell tissue and organ cult. 49; 23 – 27.
Haque, M.S, T Wada and K. Hattori. 1997. High frequency shoot regeneration and plantlet formation from root tip of garlic. Plant Cell. Tissue Org. Cult. 50; 87 – 93
Hu G, Dong Y, Zhang Z, fan X, Ren F and Zhou J. 2015. Virus elimination from in vitro apple by thermotheraphy combined with chemotheraphy. Plant Cell Tissue and organ Culture. 121 (2); 435 - 443.
Kapoor R; Nasrin. SA; Mahmoduz Zafar and Mujib. A. 2011. Establishment of efficient methods for callus culture and shoot regeneration of local Indian garlic (var. Yamum Safed). J. of Ecobio technology. 3 (2); 14-17.
Khar A; R.D. Bhutani; N Yadau; V.K. Chowdhury. 2005. Effect of explants and genotype on callus culture and regeneration in onion (Allium cepa L). Akdeniz Univ.Ziraat Fak. Dergisi. 18 (3); 397 – 401
Koch, M; Tanami Zand Salomon R. 1995. Improved regeneration of shoots from garlic callus. Hort Sci 30; 378 -379
Lee SY; Kim HH; Kim YK: Park NI and Park. SU 2009. Plant regeneration of garlic (Allium sativum L) via somatic embryogenesis. Sci Res. And Essay. 4 (13); 1569-1574.
Moriconi, D; Conci, V.C; Nome, S.F 1990. Rapid Multiplication of Garlic (Allium sativum L) in vitro. Phyton 51: 145-151.
Ohta S; Kumiga T; Nishikawa F; Yamasaki A: EndoT; Iwani. T and Yoshida T. 2011; Evalution of novel antiviral agent in the elimination of Satsumo Dwarf (SDV) by micrografting citrus. J. Japan Soc. Hort. Sci. 80; 145 – 149.
Roksana R.; MF Nature; R. Islam and MM Hossai. 2002. In vitro bulblet formation from shoot apex in garlic (Allium sativum L). Plant Tissue Culture. 12; 11-17.
Shen, Y; M.E.kane and J. Chen. 2008. Effects of genotype explants sourse and plant growth regulatots on indirect shoot organogenesis in Dieffenbachia cultivars. In vitro Cell. Dev. Biol. Plant. 44; 282 – 288.
Tan R; Wang L; Hong Ni; Goloping W; 2010. Enhanced efficiency of virus eradication following thermotheraphy of shoot tips cultures of pear. Plant Cell Tissue Organ Cult. 101; 229 – 235.
Walkey D.G.A; Webb M.J.W; Bold C.J; Miller A. 1987. Production of virus free garlic (Allium sativum L) and shallot (Allium ascolonicum L) by meristem tip culture. J. Hortic. Sci. 62; 211 – 219.
Waswa.M, Kahuhenzie R and Ochwa-Seemakula M, 2017. Effect of thermotheraphy duration, virus type and cultivar interaction on elimination of potato virus Y and S infected seed stocks. African J of plant sci. 11 (30); 61 – 70
Zaitlin M and Palukaitis 2000. Advances in understanding plant viruses and virus diseases. Am Dev Phytophatol. 38; 117 - 143.