INOCULATION TECHNIQUE OF COLLETOTRICHUM GLOEOSPORIOIDES AFFECTS Phyllanthus niruri L. GROWTH AND BIOACTIVE COMPOUND SYNTHESIS

Irna Surya Bidara; Siti Himawati; Eka Nurhangga; Yomi Guno; Winda Nawfetrias

doi:10.15575/gdcs.v66i.3414

Authors

Irna Surya Bidara National Research and Innovation Agency (BRIN), Indonesia
Siti Himawati National Research and Innovation Agency (BRIN), Indonesia
Eka Nurhangga National Research and Innovation Agency (BRIN), Indonesia
Yomi Guno National Research and Innovation Agency (BRIN), Indonesia
Winda Nawfetrias National Research and Innovation Agency (BRIN), Indonesia

DOI:

https://doi.org/10.15575/gdcs.v66i.3414

Keywords:

Endophytic fungi, Inoculation, Phyllanthus niruri, Planting medium, Seed

Abstract

Colletotrichum gloeosporioides, an endophytic fungus, significantly enhances growth, defense against pathogens, and remediation of environmental stress in medicinal plants through induced resistance and phytohormone synthesis. The application technique affects the fungi's effectiveness in stimulating growth and inducing bioactive compounds in plants. Phyllanthus niruri is an Indonesian medicinal plant with bioactive compounds like flavonoids, alkaloids, terpenoids, lignans, polyphenols, tannins, and saponins. They offer health benefits like antioxidant, anti-inflammatory, analgesic, and antiviral effects. However, research on Phyllanthus-endophytic fungi from the Colletotrichum has not been reported. This study proposes that the application technique used for C. gloeosporioides affects the fungi's effectiveness in stimulating plant growth and inducing beneficial bioactive compounds. We experimented during 2022 and 2023 at an experimental greenhouse with two different methods of fungi inoculation: seed and planting medium. The study reveals that C. gloeosporioides inoculation impacts P. niruri growth differently, with seed inoculation promoting growth and planting medium inoculation potentially hindering it. Metabolic profiling through clustering analysis indicates that fungal inoculation significantly alters the plant's chemical composition, particularly increasing the abundance of specific alkenes, suggesting activation of defense mechanisms involving very long-chain fatty acids. It highlights the critical role of the inoculation method in shaping plant-microbe interactions and influencing the production of pharmacologically relevant compounds like steroids, diketones, alkanes, and alkenes, which may serve in the plant's defense responses and have beneficial impacts on human health. Therefore, optimizing inoculation strategies and understanding the underlying biological mechanisms are crucial for harnessing the potential benefits of microbial inoculants in agriculture and biotechnology.

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Author Biographies

Irna Surya Bidara, National Research and Innovation Agency (BRIN)

Research center for Horticulture, Research Organization for Agriculture and Food

Siti Himawati, National Research and Innovation Agency (BRIN)

Research center for Horticulture, Research Organization for Agriculture and Food

Eka Nurhangga, National Research and Innovation Agency (BRIN)

Research center for Horticulture, Research Organization for Agriculture and Food

Yomi Guno, National Research and Innovation Agency (BRIN)

Research center for Aeronautics Technology, Research Organization for Aeronautics and Space

Winda Nawfetrias, National Research and Innovation Agency (BRIN)

Research center for Horticulture, Research Organization for Agriculture and Food

References

Aleynova, O. A., & Kiselev, K. V. (2023). Interaction of Plants and Endophytic Microorganisms: Molecular Aspects, Biological Functions, Community Composition, and Practical Applications. Plants, 12(4), 714. https://doi.org/10.3390/plants12040714

Basu, S., & Groot, S. P. C. (2023). Seed Vigour and Invigoration. In M. Dadlani & D. K. Yadava (Eds.), Seed Science and Technology: Biology, Production, Quality (pp. 67–89). Springer Nature. https://doi.org/10.1007/978-981-19-5888-5_4

Dudeja, S. S., Giri, R., Saini, R., Suneja-Madan, P., & Kothe, E. (2012). Interaction of endophytic microbes with legumes. Journal of Basic Microbiology, 52(3), 248–260. https://doi.org/10.1002/jobm.201100063

Indira, A., Shahar, B., Joshi, B., & Chongtham, N. (2024). Assessment of bioactive compound variations and in-vitro and in-vivo antioxidant activity in edible fresh and processed Bambusa nutans shoot through FTIR, GC/MS and HPLC analyses. Food Chemistry, 452, 139552. https://doi.org/10.1016/j.foodchem.2024.139552

Joseph, D. D., Veerasamy, K., & Siva Singaram, S. (2016). Identification of bioactive compounds by gas chromatography-mass spectrometry analysis of syzygium jambos (l.) Collected from western ghats region coimbatore, tamil nadu. Asian Journal of Pharmaceutical and Clinical Research, 10(1), 364. https://doi.org/10.22159/ajpcr.2017.v10i1.15508

Kalimuthu, A. K., Parasuraman, P., Sivakumar, P., Murugesan, S., Arunachalam, S., Pandian, S. R. K., Ravishankar, V., Ammunje, D. N., Sampath, M., Panneerselvam, T., & Kunjiappan, S. (2022). In silico, in vitro screening of antioxidant and anticancer potentials of bioactive secondary metabolites from an endophytic fungus (Curvularia sp.) from Phyllanthus niruri L. Environmental Science and Pollution Research, 29(32), 48908–48925. https://doi.org/10.1007/s11356-022-19249-0

Kumar, V., & Prasher, I. B. (2023). Phosphate solubilization and indole-3-acetic acid (IAA) produced by Colletotrichum gloeosporioides and Aspergillus fumigatus strains isolated from the rhizosphere of Dillenia indica L. Folia Microbiologica, 68(2), 219–229. https://doi.org/10.1007/s12223-022-01004-0

Kuźniar, A., Włodarczyk, K., & Wolińska, A. (2019). Agricultural and Other Biotechnological Applications Resulting from Trophic Plant-Endophyte Interactions. Agronomy, 9(12), 779. https://doi.org/10.3390/agronomy9120779

Li, X.-Z., Song, M.-L., Yao, X., Chai, Q., Simpson, W. R., Li, C.-J., & Nan, Z.-B. (2017). The Effect of Seed-Borne Fungi and Epichloë Endophyte on Seed Germination and Biomass of Elymus sibiricus. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.02488

Lopes, M. J. dos S., Dias-Filho, M. B., & Gurgel, E. S. C. (2021). Successful Plant Growth-Promoting Microbes: Inoculation Methods and Abiotic Factors. Frontiers in Sustainable Food Systems, 5. https://doi.org/10.3389/fsufs.2021.606454

Nawfetrias, W., Nurhangga, E., Reninta, R., Chotimah, S., Bidara, I. S., Maretta, D., Devy, L., Esyanti, R. R., & Faizal, A. (2023). Metabolite profiling of the medicinal herb Phyllanthus niruri L. under drought stress. IOP Conference Series: Earth and Environmental Science, 1255(1), 012046. https://doi.org/10.1088/1755-1315/1255/1/012046

Ochieng Nyalo, P., Isanda Omwenga, G., & Piero Ngugi, M. (2022). GC-MS Analysis, Antibacterial and Antioxidant Potential of Ethyl Acetate Leaf Extract of Senna singueana (Delile) Grown in Kenya. Evidence-Based Complementary and Alternative Medicine: eCAM, 2022, 5436476. https://doi.org/10.1155/2022/5436476

Patil, K., & Singh, D. (2022). GC-MS Analysis of fresh water Cylindrospermum sp. PCC518, Cylindrospermum sp. PCC 567 ethanol and hexane extracts. International Journal of Herbal Medicine, 10, 15–25.

Rhetso, T., Shubharani, R., Roopa, M. S., & Sivaram, V. (2020). Chemical constituents, antioxidant, and antimicrobial activity of Allium chinense G. Don. Future Journal of Pharmaceutical Sciences, 6(1), 102. https://doi.org/10.1186/s43094-020-00100-7

Rojas, E. I., Rehner, S. A., Samuels, G. J., Van Bael, S. A., Herre, E. A., Cannon, P., Chen, R., Pang, J., Wang, R., Zhang, Y., Peng, Y. Q., & Sha, T. (2010). Colletotrichum gloeosporioides s.l. Associated with Theobroma cacao and other plants in Panamá: Multilocus phylogenies distinguish host-associated pathogens from asymptomatic endophytes. Mycologia, 102(6), 1318–1338. https://doi.org/10.3852/09-244

Seth, T., Asija, S., Umar, S., & Gupta, R. (2024). The intricate role of lipids in orchestrating plant defense responses. Plant Science: An International Journal of Experimental Plant Biology, 338, 111904. https://doi.org/10.1016/j.plantsci.2023.111904

Sharma, M., & Kulshrestha, S. (2015). Colletotrichum gloeosporioides: An Anthracnose Causing Pathogen of Fruits and Vegetables. Biosciences Biotechnology Research Asia, 12(2), 1233–1246. https://doi.org/10.13005/bbra/1776

Suyambumani, S., Pandiyan, J., Wong, L. S., Djearamane, S., Mathanmohun, M., & Sagadevan, S. (2024). Phytochemical analysis and antimicrobial potential of Bauhinia tomentosa leaf extracts. Journal of Phytology, 41–48. https://doi.org/10.25081/jp.2024.v16.8456

Tefera, T., & Vidal, S. (2009). Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana. BioControl, 54(5), 663–669. https://doi.org/10.1007/s10526-009-9216-y

Tripathiathi, N., Kumar, S., Singh, R., Singh, C. J., Singh, P., & Varshney, V. K. (2013). Isolation and Identification of γ-sitosterol by GC-MS from Roots of Girardinia heterophylla. Oriental Journal of Chemistry, 29(2), 705–707.

Wei, L., Ran, J., Li, Z., Zhang, Q., Guo, K., Mu, S., Xie, Y., Xie, A., & Xiao, Y. (2024). Chemical Composition, Antibacterial Activity and Mechanism of Action of Fermentation Products

from Aspergillus Niger xj. Applied Biochemistry and Biotechnology, 196(2), 878–895. https://doi.org/10.1007/s12010-023-04577-9

Zouaghi, N., Houda Bensiradj, N. E., Cavaleiro, C., Nadjemi, B., & Trari, M. (2021). Phytochemical Study and Antibacterial Effects of Fraxinus angustifolia Vahl (Algeria): Experimental and Computational Investigations. Waste and Biomass Valorization, 12(7), 3605–3616. https://doi.org/10.1007/s12649-020-01240-w