INDUKSI TUNAS ANGGREK (Dendrobium sp) Var. Kumala MENGGUNAKAN BAP (6-Benzyl Amino Purine) DAN AIR KELAPA SECARA IN-VITRO
Article Sidebar
CRD,
coconut water,
Dendrobium,
MS
Main Article Content
Abstract
Orchid plants are one type of ornamental plant that has a unique flower beauty and a long flower life compared to other flower plants. The beauty and attractiveness of orchids lies in the various shapes and colors of their flowers. because this Dendrobium orchid has the potential as an ornamental plant so that it is widely developed and propagated in Indonesia because the climate is very suitable for growing orchids. This study aimed to determine the effect of BAP (6-Benzyl amino purine) and coconut water on the growth of orchid explants in vitro and to determine the optimum concentration of the combination of BAP and coconut water on the growth of orchid explants in vitro. The method used is the Completely Randomized Design (CRD) method using MS media, there are 10 concentration variations where 1 control concentration and 9 concentrations of BAP and coconut water combination. Each treatment consisted of 3 repetitions. Thus, 30 experimental units were obtained. The results showed that MS media using a combination of 1.5 ppm BAP and 200 ml of coconut water could trigger the growth of leaves, 0.5 ppm BAP treatment plus 150 ml of coconut water could trigger stem elongation and shoot number, while the control treatment can trigger the growth of the number of roots. The survival percentage is 100% where none of the 30 ripening bottles is contaminated.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Akbar M, A., Faridah, E., Indrioko, S., & Herawan, T. (2017). Induksi Tunas, multiplikasi dan Perakaran Gyrinops versteegii (Gilg.) Domke secara in vitro. Jurnal Pemuliaan Tanaman Hutan, 11(1), 1–13.
Adawiyah, A. (2011). Pengaruh Giberelin (GA3) dan Air Kelapa terhadap Organogenesis Kultur Jaringan Krisan (Chrysanthemum morifolium Ram.) varietas Mustika Kaniya secara In Vitro. Skripsi. Bandung: Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Gunung Djati.
Alfrida, Maninggolang. (2018). Pengaruh BAP (Benzyl amino purine) dan Air Kelapa Terhadap Pertumbuhan Tunas Pucuk dan Kandungan Sulforafan Brokoli (Brassica oleracea l. var. Italica plenck) Secara In-Vitro. Agri-SosioEkonomi Unsrat, 14(1), 585-596.
Fitriani, H. (2008). Kajian Konsentrasi BAP dan NAA Terhadap Multiplikasi Tanaman Artemisia annua L. Secara In Vitro. Universitas Sebelas Maret, 53(9), 1689–1699.
Goerge E. F. & P. D. Sherrington. (1984). Plant propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. England: Exegenetic Ltd. Eversley
Henuhili, V., (2005). Tanaman Transgenik dan Pemenuhan Kebutuhan Pangan. Seminar Nasional Pendidikan, Penelitian, dan Penerapan MIPA, B.150-B.115.
Inpeuy, K., Chaemalee, S., & Te-chato, S. 2011. Cytokinins and coconut water promoted abnormalities in zygotic embryo culture of oil palm. Songklanakarin Journal of Science and Technology, 33 (6).
Kartiman, R., Sukma, D., Aisyah, S. I., & Purwito, A. (2018). Multiplikasi in-vitro anggrek hitam (Coelogyne pandurata lindl.) pada Perlakuan Kombinasi NAA dan BAP. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 5(1), 75.
Marlina, N. (2004). Teknik Modifikasi Media Murashige dan Skoog (MS) untuk Konservasi In Vitro Mawar. Bulletin Teknik Pertanian, 9(1), 4–6.
Novianto. (2012). Prospek pengembangan usaha anggrek berbasis sumber daya lokal. Prosiding Seminar Nasional Anggrek. Balai Penelitian Tanaman Hias. Puslitbang Hortikultura-Balitbang Pertanian.
Putri, D. A. (2016). Pertumbuhan Planlet Anggrek Dendrobium sylvanum “Flava” pada Berbagai Jenis Media. Diploma thesis. Bandung: UIN Sunan Gunung Djati Bandung.
Sandra, E. (2003). Kultur Jaringan Skala Rumah Tangga. Jakarta: Agromedia Pustaka.
Sarwono, B. (2002). Menghasilkan anggrek potong kualitas prima. Jakarta: Agro Media Pustaka.
Wattimena, G. A., L. W. Gunawan, N. A. Mattjik, E. Syamsudin, N. M. A. Wiendi, & A. Ernawati. (1992). Bioteknologi Tanaman. Bogor: Pusat Antar Universitas Bioteknologi, Institut Pertanian Bogor.
Widiastoety, d. & Purbadi. (2003). Pengaruh Bubur Ubi Kayu dan Ubi Jalar terhadap Pertumbuhan Plantlet Anggrek Dendrobium. Jurnal Hortikultural 13(1), hal 1-6.
Yunita, R. (2011). Pengaruh Pemberian Urine Sapi, Air Kelapa, dan Rootone- F Terhadap Pertumbuhan Setek Tanaman Markisa (Passiflora edulis var. flavicarpa). Skripsi. Padang: Fakultas Pertanian Universitas Andalas.